A MINIATURE SPR BIOSENSOR FOR THE DETECTION OF 3-NITROTYROSINE
3-Nitrotyrosine is a biomarker for nitric oxide-mediated tissue injury. It is found in human plasma and urine. A Spreeta 2000 liquid index sensor was used to developed a surface plasmon resonance (SPR)-based biosensor for detection of 3-nitrotyrosine. Due to its small molecular weight, the direct binding of 3-nitrotyrosine molecules to SPR biosensor surface will not give significant index change that is useful for its quantitation. Thus, an inhibition immunoassay was used for the measurement of 3-nitrotyrosine concentration with this SPR biosensor. The change in refractive index was found to be linear with logarithmic target concentration in the range of 0.14 to 5. 31μg/mL. The detection limit was 0. 031μg/mL. The sensing surface of this SPR biosensor could be regenerated for consecutive analysis of urine samples.
3-Nitrotyrosine Surface Plasmon resonance (SPR) Biosensor Inhibition immunoassay
Lu Hua Jin Jing Tao Yi
State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
168-173
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）