APPLICATION OF REAL-TIME QUANTITATIVE PCR TO ASSESS ENVIRONMENTAL ABUNDANCE OF TOXIC MCROCCYSTIS IN TAIHU LAKE
Environmental abundance of toxic Microcystis in Taihu Lake with real-time quantitative PCR (RTQ-PCR) was studied in this paper. The standard curves of Cyanobacteria, Microcystis and toxic Microcystis were established by RTQ-PCR, respectively. The environmental abundance of Microcystis and toxic Microcystis was demonstrated by Ct ratio of Microcystis and Cyanobacteria and Ct ratio of toxic Microcystis and Cyanobacteria, respectively. The results showed that the ranges of Cyanobacteria, Microcystis and toxic Microcystis were 106 -1011gene copies/μL (R2=0. 994 1) , 104 -109gene copies/μL (R2=0. 993 6) and 102 -105gene copies/μL (R2=0. 995 1) , respectively. During May, June and July of 2009, Algal density in Taihu Lake was 1. 5 × 105, 2. 9 × 105, 1. 8 × 106 cells/mL. The environmental abundance of Microcystis was about 90％ and the abundance of toxic Microcystis was up to over 40％ in the lake. It is proved that RTQ-PCR is a useful tool for the rapid quantification of environmental abundance of Cyanobacteria, Microcystis and toxic Microcystis and the main Microcystis of Taihu Lake bloom was toxic Microcystis.
Real-time quantitative PCR tozic Microcystis, abundance Taihu Lake.
Li Xiao Q. Yang Fei Yuan Jun Yin Li H. Liu Ran Liang Ge Y. Li Yun H. Pu Yue P.
Department of Occupational and Environmental, Health, School of Public Health, Southeast University, College of Civil Engineering, Southeast University, Nanjing, 210096, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
196-199
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）