COMPARISON OF THREE WHOLE GENOME AMPLIFICATION (WGA) METHODS OF GENOMIC DNA FROM ESCHERICHIA COLI
In many large genetic studies, the amount of available DNA can be one of the criteria for selecting samples for study. Being well-known, DNA sample preparation is time-consuming and laborious, even it seems unanswered when the type and amount of sample available is a limiting factor, either due to degradation or low copy number. To solve these problems, Whole genome amplification (WGA) , based on DNA-strand displacement, is an option to amplify DNA in not-yet longer time reaction. The DNA amplification is non-PCR based and uses Φ29 DNA polymerase and modified random hexamer primers for unbiased whole genome amplificatioa There are various WGA kits which have been developed, for example the Repli-g Mini kit (Qiagen) and the GenomiPhi kit (GE Healthcare). So, it is of great important to select a perfect Kit suited for our research. In this study, after comparison of three WGA kits, there are the differences illustrated that the WGA product amplified from Escherichia coli genomic DNA is representative of the different amplification efficiency.
Whole Genome Amplification (WGA) Amplification efficiency Escherichia coli
Ma Chao Li Zhiyang Li Xiaolong He Lei He Nongyue
State Key Laboratory of Bioelectronics,School of Biological Science and Medical Engineering, Southea State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southe
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
229-231
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）