COMPLETELY STABLE STREPTAVIDIN IMMOBILIZED ON MAGNETIC BEADS IN TERMS OF THERMAL TREATMENT CYCLES FOR ADVANCED GENOME SEQUENCING

摘要：

In the present research, we succeeded to improve the heat resistant property of streptavidin immobilized on magnetic beads (SA - MB) by addition of specific additives into buffer solution. The reactivity of SA - MB was investigated in terms of the hybridization efficiency of complementary single stranded DNA (complementary-ssDNA) with biotinylated single stranded DNA (biotin-ssDNA) immobilized on SA- MB surface. The addition of ammonium sulfate into the buffer solution drastically improved the heat resistant property of SA - MB after heat-treatment at 95°C for 5 min. The hybridization reactivity was retained by the addition of ammonium sulfate even after three cycles of heat-treatment.

关键词： Magnetic beads streptavidin heat-induced inactivation DNA hybridization PCR

作者: Masaki Kubota Ketaro oshimoto Yukio Nagasaki

作者单位: Graduate School of Pure Applied Sciences, University of Tsukuba Graduate School of Pure Applied Sciences, University of Tsukuba Tsukuba Research Center for Interdis Graduate School of Pure Applied Sciences, University of Tsukuba Tsukuba Research Center for Interdis

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 232-233

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）