CONSTRUCTION OF A RECOMBINANT LENTTVIRAL VECTOR CONTAINING MIR-210 AND ITS STABLE EXPRESSION IN MEC

摘要：

Recent research indicates that miR - 210 is involved in various biological processes of blood vessel endothelial cell. In present study, we report the construction of recombinant lentivirus miR210 expression vector and its stable expression of miR- 210 in transfected human microvascular endothelial cells (hMECs). The precursor of human miR - 210 gene was amplified by PCR and the PCR products were linked with eukaryotic expression vector pGCSIL-GFP. Through packaging by 293T cell, the recombinant lentiviral expression vector of miR - 210 and its controls were transfected to hMECs. Fluorescence detection showed that fluorescence intensity of GFP was highest between 48 and 72 hours post-infection, and qRT-PCR showed an increase in miR - 210 expression in recombinant lentivirus transfected cells compared with the expression in its controls. The recombinant lentiviral expression vector of miR- 210 was constructed successfully, which may facilitate further study of blood vessel endothelial cell.

关键词： miR-210 hMECs lentiviral vector

作者: Lou Yuanlei Deng Zhifeng Gao Fangliang Ruan Qiangfang Xie An Guo Fei Wang Yang

作者单位: Institute of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, Institute of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006,China Burns Institute of the First Affiliated Hospital of Nanchang University, Nanchang330006, China

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 239-242

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）