CONSTRUCTION OF HIGH-THROUGHPUT SINGLE CELL-BASED SYSTEM FOR SCREENING OF INTERACTIVE MOLECULES

摘要：

To identify interactive molecules of proteins is an important process which leads to clarification of physiological characteristics or development of novel drugs. However, this process has many difficulties because we have to screen many molecules. So, the screening often becomes a rate-determining step. In this study, we aim at constructing high-throughput single cell-based system for screening of interactive molecules. We developed yeast cell surface display, yeast single cell chip, and automatic single-cell pick-up machine to screen many molecules comprehensively and easily. We constructed a combinatorial yeast library in which each yeast cell displays different peptide on its cell surface, and we screened yeast cells which interacted with a model protein, neurolysin, a close relative of cancer matrix metalloproteinase (MMP) - 2/- 9, using yeast single-cell chip and automatic single-cell pick-up machine. We succeeded to obtain cells displaying peptide which specifically interacted with neurolysin.

关键词： Yeast cell surface display yeast single-cell chip automatic single-cell pick-up machine neurolysin

作者: Wataru Aoki Kouichi Kuroda Mitsuyoshi Ueda

作者单位: Division of Applied Life Sciences,Graduate School of Agriculture, Kyoto University, Kitashirakawa-oi Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-o Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-o

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 243-244

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）