DEVELOPMENT OF MULTI-FUNCTIONAL PROBES FOR GENE XPRESSION ANALYSIS AT SIGLE CELL LEVEL
Single-cell mRNA analysis has been performed by electrical cell lysis technique with Pt-ring electrode-capillary probe and RT real-time PCR. Four different hose-keeping genes were detected from single cell and it was found that our multi-functional probe technique allowed quantitative single-cell mRNA analysis at 10 copies/cell. Single-cell mRNA analysis in reporter system has been also performed to validate our mRNA analysis technique by using GFP reporter system as a model. Messenger RNA and protein expressed from identical individual cells were analyzed with real-time PCR and fluorescence microscope, respectively. Basically, the fluorescence intensity was linear to the GFP mRNA copy number of the individual transfected HeLa cell.
Single-cell analysis RT-PCR real-time PCR scanning probe microscopy cell manipulation
Hitoshi Shiku Takeshi Yamakawa Daisuke Okazaki Genki Saito Yasumoto Date Kosuke Ino Tomokazu Matsue
Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Sendai 980-8579, Japan
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
261-263
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）