DIFFERENTIAL EXPRESSION OF HUMAN TELOMERASE REVERSE TRANSCRIFIASE β-SITE REMAINING LTERNATIVE SPLCING VARIANTS (HTERT β+ ASV) IN GASTRIC CANCER AND PRECANCEROUS LESIONS
Objective:To investigate the changes of hTERT alternative splicing variants pattern in gastric cancer, precancerous lesions and normal gastric mucosa tissue. Methods: Three alternative splicing sites (α, β, γ) were selected using Semi-nested RT-PCR and SYBER Green real-time PCR. Telomerase enzyme activity was evaluated associated with the different hTERT ASVs. Results: The positive rates of β+ ASVs were 11. 1％ in normal mucosa, 40. 0％ in precancerous lesions and 94. 7％ in gastric cancer (P<0. 05). SYBR Green real-time RT-PCR showed that the expression level of β + ASV was 6. 99 times higher in gastric cancer than in precancerous lesions. Further, increased telomerase enzyme activity was only associated with expression of the full-length hTERT isoform. Conclusion: The gene expression patterns of hTERT alternative splicing variants may provide some useful information for diagnosis of gastric cancer and precancerous lesions.
Alternative splicing gastric cancer precancerous lesion telomerase reverse transcriptase
Geng Xin Xu Jinheng Wang Yuchuan Wang Fei Zhang Weiming
Department of Biochemistry, Tianjin Medical University, Tianjin, China Department of Pathology, Tangshan workers hospital of HeBei Meidical University, Tangshan, China Tianjin Institute of Ophthalmology, Tianjin, China Department of Neurology, General Hospital, Tianjin Medical University, Tianjin, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
264-268
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）