EXPRESSION ANALYSIS OF THE HIGH-AFFINITY NITRATE RANSPORTERS IN RICE:SPATIAL EXPRESSION AND REGULATION BY NITRATE
Expression of OsNAR2. 1 and OsNRT2 genes in rice were investigated in different nitrogen supplies by relative quantitative RT-PCR and promoter fusion with GUS reporter gene. The results showed that OsNAR2. 1 and OsNRT2. 1 were regulated up by nitrate but significantly down by ammonium. Further more OsNRT2. 2 was decreased by ammonium but not very significantly. It was clear that the responses of OsNRTZ. 3a to nitrate and ammonium were different from OsNRT2. 3b, even though they were, basically to say, regulated by a same promoter in genomic sequence. And OsNRT2. 4 was mainly expressed in shoot not in root. The expression patterns of two OsNAR2s and four OsNRT2s promoter-GUS showed wide variation in different rice organs. In the end the possible function and regulation mechanism of OsNAR2. 1 and OsNRT2s was discussed in this paper.
NRT2 NAR2 GUS Oryza sativa.
Feng Huimin Yan Ming Li Baozhen Fan Xiaorong Xu Guohua
College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
289-293
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）