EXPRESSION CHANGES OF MIR-210 AND ITS TARGET GENE EPHRINA3 IN ISCHEMIC CORTEX AFTER STROKE IN ADULT RAT
The timely revascularization in ischemic region after stroke is crucial for recovery of neurological function but the regulatory mechanism of this process is still not clear. Mir - 210 is a hypoxia-specific microRNA, and evidence showed that overexpression of mir - 210 in endothelial cell response to hypoxia could stimulate endothelial cell migration and capillary formation by blocking its target gene ephrinA3 protein expression. Whether mir - 210 is involved in the regulation of angiogenesis after brain ischemia has not been reported yet. In this study, we investigated the expression changes of mir - 210 and ephrinA3 in adult rat ischemic brain cortex after stroke. Results showed that mir- 210 expression was significantly upregulated in ischemic cortex at 1,3 and 7 days after stroke; furthermore, a gradually decreased ephrinA3 protein level was simultaneously detected in ischemic cortex at 1, 3 and 7 days post-ischemia. We concluded that the hypoxic conditions in ischemic cortex could up or downregulate mir- 210 and ephrinA3 expression respectively, suggesting a potential role of mir- 210 in ischemia-induced angiogenesis after stroke.
Angiogenesis brain ischemia microRNA microRNA210 ephrinA3 bloodvessel
Gao Faliang Wang Yang Lou Yuanlei Ruan Qiongfang Lv Shigang Hu Yawei Pan Changfu Deng Zhifeng
Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, Institute of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University, Nanchang330006, C
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
294-298
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）