EXPRESSION OF LHC GENES INVOLVED IN PS I AND PSⅡ IN MOSO BAMBOO SEEDLINGS
Isolation and expression of the light-harvesting chlorophyll a/b binding protein (Lhc) genes from moso bamboo were performed in this study. The full-length of two Lhca genes PeLhca01 and PeLhca02 involved in PS I and two Lhcb genes Pelhcb3 and Pelhcbl involved in PS II were cloned by RT-PCR and RACE methods. Mose bamboo seedlings were treated with different light intensity. Fluorescence parameters of Moso bamboo seedlings high light intensity changed in contrast to normal light. The four Lhc genes tissue-specific, temporal and spatial expression were analyzed. The expression level of the two Lhca genes decreased obviously in high intensity of light treatment, while increased significantly in low intensity of light treatment. The expression level of the two Lhcb genes decreased obviously. It could be infered that two Lhcb genes be related with photoinhibition and two Lhca genes be related with the capacity of harvesting light. In different vegetative organs two Lhca genes have their expression profiles.
Lhca genes Lhcb genes intensity of light real-time PCR gene ezpression Moso bamboo
Tang Wenli Peng Zhenhua Gao Jian Zhang Zhijian Zhang Yan
International Centre for Bamboo and Rattan, Key Laboratory of Bamboo and Rattan Science and Technology of the SFA, Beijing 100102, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
299-303
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）