FISHING OUT THE PARTIALLY FOLDED INTERMEDIATES IN GUANIDINE HYDROCHLORIDE INDUCED UNFOLDING-REFOLDING PATHWAY OF TRYPSIN

摘要：

GdmCl (guanidine hydrochloride)induced unfolding and refolding of porcine pancreas trypsin have been studied by various physicochemical methods including near and far UV CD (Circular dichroism) spectrum, intrinsic fluorescence spectroscopy (intensity, emission maximum wavelength, tryptophans anisotropy etc. ) as well as ANS (8 - anilino - 1 - naphthalene-sulfonic acid) fluorescence. In our present study, the formation of two unfolding intermediates has been established. They were further confirmed by phase diagram approach. Descriptions of protein unfolding-refolding pathway and the intermediates conformations have also been given.

关键词： Trypsin partially folded intermediates CD spectrum fluorescence spectroscopy phase diagram

作者: Qu Peng Lu Hua Ding Xiaoyu Tao Yi Lu Zu Hong

作者单位: State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering,Southeast State Key Laboratory of Bioelectronics, School of Biological Science & Medical Engineering,Southeast

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 308-312

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）