FLUORESCENCE RESONANCE ENERGY TRANSFER BASED QUANTUM DOT-DNA CONJUGATE FOR SENSITIVE DETECTION OF EPATITIS B VIRUS DNA

摘要：

Persistent infection with hepatitis B virus ( HBV) is a major health problem worldwide that may lead to chronic hepatitis, cirrhosis and primary liver cancer. In this paper, we report a QDs-DNA nanosensor based on fluorescence resonance energy transfer (FRET) for sensitive and specific detection of HBV DNA hybridization where the separation of un-ligated DNA strands from hybridized system are unnecessary. Experimental results showed that the proposed approach was suitable for label-free detection of specific DNA targets at low DNA concentration. The practical utility of the proposed method was demonstrated in the detection of synthetic 30-mer oliginucleotide targets derived from the hepatitis B virus with a sensitivity of 35 nM by a multilabel plate assay, which might make the application of the proposed method possible due to the simplicity and of the multilabel counter.

关键词： Quantum dots (QDs) Cy5 Hepatitis B virus (HBV) DNA Fluorescence resonance energy transfer (FRET) Hybridization

作者: Wang Xiang Wang Yi Guo Qing C. Zhong Xin H. Mao Hong J. Zhao Jian L.

作者单位: Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, Department of Chemistry, East China University of Science and Technology, Shanghai, 200237, China

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 313-317

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）