FLUORESCENT DETERMINATION OF PYROPHOSPHATE WITH A CONJUGATED COMPOUND

摘要：

The fluorescence intensity of conjugated compound, tri -9,9 - bis(3 - diethoxyl phosphorylpropyl) fluorene (TDPF) can be strongly quenched by Fe3+. Addition of pyrophosphate (PPi) into TDPF-Fe3+ system induces efficient recovery of the TDPF fluorescence intensity due to the complexation between PPi and Fe3+. Based on this phenomenon, the PPi can be conveniently and sensitively detected in the concentration range of 0. 5 - 10 μM with high selectivity. The proposed method for PPi detection offers the potential for detecting sequence-specific nucleic acid and studying biological processes.

关键词： Pyrophosphate conjugated compound fluorescence recovery

作者: Li Zhengping Wang Xiaoke Gao Baoxiang Liu Chenghui

作者单位: Key Laboratory of Medicine Chemistry and Molecular Diagnosis, Ministry of Education College of Chemistry and Environment Science, Hebei University, Baoding 071002, China

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 318-319

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）