GENE EXPRESSION PROFILING IN BREAST CANCER BY SRPP METHOD
Background : Gene expression profiling has provided us with insight into the prognostic of breast cancer and led to the development of molecular gene signature models designed to aid in clinical decision-making. We sought to develop a quantitative analysis of the relative expression levels of a target gene from tumor tissues and normal tissues in a single assay. Methods: Sequence-tagged reverse-transcription polymerase chain reaction coupled with pyrosequencing (SRPP) was used for comparing prognostic gene relative expression levels in breast tumor. In the pyrogram, the sequence represents the gene source and the peak intensity represents the relative expression level of the gene in corresponding source.Results:The expression levels of ten kinds of prognostic marker genes (AL080059, MMP9, EXT1, ORC6L, AF052162, C9orf30, FBXO31, IGFBP5, ESM1 and RUNDC1) among the breast tumor tissues and normal tissues in the patients were accurately detected (n=3). The accuracy of SRPP was contrasted to the result of real-time PCR (n=3).Conclusions: The results show that SRPP method is reliable and reproducible in quantitatively comparing gene expression levels among different sources at a low cost. It can be apply to the prognostic gene relative expression assay in breast cancer.
Gene-ezpression profiling Breast cancer SRPP Pyrosequencing
Song Qinxin Jing Hua Zhou Guohua
Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China Huadong Research Institute for Medicine and Biotechnics, Nanjing 210002, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
326-331
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）