HCV CORE FRAME SHIFT PROTEIN, F PROTEIN, SHARES THE REPRESSION OF P21 PROMOTER ACTIVITY WITH CORE PROTEIN
Hepatitis C virus (HCV) core protein was proven previously to repress transcription of the universal cyclin-dependent kinase (CDK) inhibitor p21 gene, as demonstrated by in vitro transient expression assays using murine fibroblasts (NIH3T3), human hepatocellular carcinoma (HepG2) and human cervical carcinoma (HeLa) cells. Researchers have found a new HCV protein, termed as F protein, which is encoded from an alternative +1 reading frame overlapping the core genomic region. In this study, we investigated whether or not F protein repressed transcription of the universal cyclin-dependent kinase inhibitor p21 gene as core protein did. From the transient reporter assays of p21 promoter, we found that F protein, similar but not the same as core protein, repressed transcriptional modulation of p21 gene. So we concluded that F protein can regulate p21 promoter, which were previously attributable to core protein expression.
Hepatitis C virus (HCV) F protein core protein p21 promoter
Kong Jing Deng Xiaozhao Zhang Yun Wang Zhongchan Chu Chunli Yu Juan Li Chunlei
Medical Institute of Nanjing Army, Nanjing 210002, China Faculty of Life Scence & Chemical Engineeri Medical Institute of Nanjing Army, Nanjing 210002, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
332-335
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）