HUMAN INDUCED PLURIPOTENT STEM CELLS EXPRESS NEURAL PROGENITOR CELL GENE
Induced pluripotent stem cells (iPS cells), a kind of pluripotent cells which derived from somatic cells through reprogramming, is an potential substitute of ES cells and become an ideal donor source of cell-based transplantation. A clinical application of human iPS cells would require the generation of donor cells for specific tissues and organs. Here we report the derivation of neural progenitor cells with differentiation potency from human iPS cells. After a 4-/4+ 5×10-7 M retinoic acid (RA) inducing period, iPS cells aggregates were plated for adherent culture. Neuroepithelial rosette construction appeared in 4 days after plating, these cells expressed neural progenitor cells gene and differentiated to β-tubulin III positive neurons. Our results indicated that human iPS cells can differentiate toward neural progenitor cells in the presence of 5×10-7 M RA The results suggested that human iPS cell was a potential source of neural progenitor cells for repairing nervous system injury.
Induced pluripotent stem cells neural progenitor cells differentiation retinoic acid
Feng Nian Hua Xie An Lou Yuan Lei Ruan Qiong Fang Guo Fei Wang Yang
Institute of Urology, the first affiliated hospital of Nanchang University, Nanchang 330006, China Burns Institute, the first affiliated hospital of Nanchang University, Nanchang 330006, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
344-347
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）