IDENTIFICATION PRES1 BINDING PROTEIN USING A PROTEOMICS STRATEGY
The PreSl region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreSl interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreSl. The interactions between PreSl and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreSl binding protein enriches our knowledge about molecular mechanism of HBV infection.
HBV PreSl Proteomics GRP75
Cui Lunbiao Qi Yuhua Shan Jun Ge Yiyue Shan Yunfeng Shi Zhiyang Qi Xian Wang Hua
Institute of Pathogen Microbiology,Jiangsu Provincial Center for Diseases Prevention and Control,Nan Institute of Pathogen Microbiology, Jiangsu Provincial Center for Diseases Prevention and Control,Na Institute of Pathogen Microbiology, Jiangsu Provincial Center for Diseases Prevention and Control,Na
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
348-352
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）