METANEPHRIC CELLS MICROENVIRONMENT INDUCES DIFFERENTIATION OF EMBRYONIC STEM CELLS TOWARD RENAL CELLS
Embryonic stem (ES) cells co-cultured with different embryonic organic cells could differentiate into a broad spectrum of specialized cell types, including hepatocyte, cardiomyocyte and alveolar epithelial type II cell types. Such ES cells derived cells can provide a valuable source of progenitor cell types, and could be a new resource of seed cells in regenerative medicine. In the present study, we imitate the embryonic kidney development microenvironment by co-culture the mouse ES cells with fetal renal cells in a dual chamber dish separated by a semipermeable membrane. During this non-direct contact co-culture procedure, nephrogenic differentiation and relative molecular markers expression in ES cells were evaluated. Immunocytochemistry analysis showed that in the co-culture group, the positive cells of Pax2 and WT1 in the EBs emerged in the 3rd day, with the prolongation of co-culture time, the positive cells number increased. The results of RT-PCR showed that the expressed genes essential for kidney development such as Pax2, WT1, Emx2, GDNF, BMP7, Nephrin, KSP and the Kidney progenitor cell marker CD24 mRNA levels in the EB cells were promoted in the metanephric cells microenvironment. With the prolonged co-culture time, the expressions of most genes mentioned above were reduced, however genes expression of Nephrin and KSP were enhanced. In conclusion, the metanephric cells microenvironment imitated by non-contact co-culture is able to induce ESCs to differentiate toward renal cells, and regulate the expression of its renal development related genes.
Embryonic Stem Cells Renal Cells Microenvironment Differentiation
Xie An Zhang Lixing Zhao Ping Lou Yuanlei Feng Nianhua Guo Fei Wang Yang
Institute of Urology, First affiliated Hospital of Nanchang University, Nanchang, 330006,China Institute of Urology, First affiliated Hospital of Nanchang University, Nanchang, 330006, China Jiangxi Academy of Science, Nanchang 330006, China Burns Institute of the First Affiliated Hospital of Nanchang University, Nanchang 330006, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
368-370
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）