Sporadic cases of oseltamivir resistant swine-origin influenza A (H1N1) viruses (S-OIVs) have been detected worldwide. It is urgent to monitor the antiviral susceptibility to neuraminidase inhibitors (NAIs) (oseltamivir). Pyrosequencing has been successfully used to monitor the M2 blocks (adamantane) and NAIs (oseltamivir) in influenza A viruses. We designed a novel labeled universal primer pyrosequencing assay for detection in S-OIVs. Thirty clinical specimens and three virus isolates collected from Jiangsu Province of China were tested and our data showed that all cases are resistance to amantadine but sensitive to oseltamivir. Comparing the sequence-specific labeled primer, the new designed universal primer can significantly improve the efficiency of detection and decrease the expense and time involved in synthesizing sequence-specific labeled primers.
Antiviral susceptibility swine-origin influenza A(H1N1) virus universal primer pyrosequencing
Qi Yuhua Cui Lunbiao Shan Yunfeng Qi Xian Ge Yiyue Shan Juh Shi Zhiyang Wang Hua Lu Zuhong
Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Institute of Pathogenic Microbiology Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Institute of Pathogenic Microbiology Key Lab of Enteric Pathogenic Microbiology, Ministry of Health, Institute of Pathogenic Microbiology State Key Lab of Bioelectronics, School of Biological Science and Medical Engineering, outheast Univ