NOTCH SIGNAL MOLECULES MEDIATE ANGIOGENESIS IN ISCHEMIC CORTEX AFTER STROKE IN RAT
It was reported that intravenous infusion of bone marrow mesenchymal stem cells (MSCs) could promote angiogenesis in ischemic areas, but the molecular mechanism is unclear. We investigated the Notch signaling pathway in regulating angiogenesis after stroke. MSCs were intravenously injected into rats 24 hours after middle cerebral artery occlusion (MCAo). Immunofluorescent staining was performed to assay angiogenesis in ischemic cortex. Immunofluorescent staining and Semi-Quantitative RT - PCR were used to detect the expression of Notchl and Hesl in ischemic cortex. Morphological image analysis revealed significant increases in numbers of microvessels and Notchl positive microvessels in the ischemic cortex of rats treated with MSCs compared with rats treated with PBS. Semi-Quantitative RT-PCR results showed that MSCs transplantation significantly raised NotchlmRNA and HeslmRNA levels in the ischemic cortex compared with cerebral control group. These data suggested that the Notch signaling pathway mediated angiogenesis in cerebral ischemia after stroke.
Notch signaling pathway bone marrow mesenchymal stem cells angiogenesis stroke
Lv Shigang Wang Yang Tu Wei Zhang Aijun Lou Yunlei Lai Xianliang Deng Zhifeng
Department of Neurosurgery, the Second Affiliated Hospital of Nanchang University Institute of Urology, Nanchang University,Nanchang 330006, China Institute of Urology, Nanchang University, Nanchang 330006, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
382-385
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）