OVEREXPRESSION OF HEPATIC NF-KB AND ITS CLINICAL IMPLICATIONS IN HBV-RELATED HEPOTOCELLUIAR CARCINOMA
Objective: To investigate the NF-κB expression in HBV-related HCC and evaluate its clinical implications. Methods NF-κB expression was detected in HCC and their corresponding tissues. Hepatic HBV-DNA was detected by in situ molecular hybridization. Total RNA were abstracted and NF-κBmRNA were amplified by real-time-PCR. The relationship was analyzed between NF-κB and HBV. Results Positive NF-κB was brown granule-like and localized in nucleus and cytoplasm in HCC with stronger expression, and only in cytoplasm in its surrounding tissues. Its incidence was 100％ in HCC, and 68. 6％ in their surrounding tissues. Significant difference was found between the two groups. None of the other variables positive relationship presented between NF-κB and differentiation grade, number or size of tumor or AFP. NF-κB in HBV-DNA-positive HCC was significantly higher than that in HBV-DNA-negative ones. Conclusion NF-κB was closely associated with the HBV-related HCC and could be a marker for development of HCC.
Hepatocellular carcinoma NF-κB Immunohistochemistry Real-time PCR HBV-DNA
Yao Dengfu Wu Wei Sai Wenli Qian Jing Li Shanshan Bian Yinzhu
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Chi Research Center of Clinical Medicine,Affiliated Hospital of Nantong University, Nantong 226001, Chin
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
396-399
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）