PROTEOMIC ANALYSIS OF RAT MYOCARDIAL INFARCTION MODEL
To identify the changes of cardiac protein expression during myocardial infarction occur, the animal model of myocardial infarction was established by surgical ligation of the left coronary artery in rats. Protein profiles of normal and infarcted tissues were investigated by 2DE: over 850 60 protein spots were detected in the silver-stained gels. The results showed that the expressions of 19 protein spots were increased and 13 protein spots were decreased in the diseased tissues when compared with normal tissues (n=5). 16 altered protein spots were subjected to mass spectrometry analysis, and 11 proteins were subsequently identified with peptide mass fingerprinting (PMF) based on matrix-associated laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching. These identified proteins were Hsp27 (2 isoforms),
Myocardial infarction Proteomics Two-dimensional gel electrophoresis Mass spectrometry
Ye Nengsheng Luo Guoan Liang Qionglin Wang Yiming
Department of Chemistry, Capital Normal University, Beijing 100048, China Institute of Biomedicine, Institute of Biomedicine, singhua University, Beijing 100084, China Institute of Biomedicine, Tsinghua University, Beijing 100084, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
410-412
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）