RAPID AND VISUAL IDENTIFICATION OF SWINE INFLUENZA H1N1 BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION
Swine influenza H1N1, which is an acute respiratory disease, is seriously threatening humans health. Nowadays, molecule diagnosis is an effective and accurate way to diagnose H1N1. Here, we developed a method based on loop-mediated isothermal amplification (LAMP) for rapidly and visually identifying swine influenza H1N1 on molecule levels. To perform highly efficient amplification, the concentrations of Mg2+, Bst DNA Polymerase, dNTPs and the ratio of outer and inner primers were optimized. The product of LAMP from the clinical sample was successfully detected by three methods including precipitation, fluorescence and electrophoresis. The results showed that the sensitivity could reach 103 copies. Therefore, our developed LAMP method can be used to rapidly and visually identify swine Influenza H1N1.
Loop-mediated isothermal amplification (LAMP) H1N1
Cheng Sijia Chu Yanan Chen Zhiyao Zhou Guohua
China Pharmaceutical University, Nanjing, China Huadong Research Institute for Medicine and Biotechn China Pharmaceutical University, Nanjing,China Huadong Research Institute for Medicine and Biotechni China Pharmaceutical University, Nanjing,China China Pharmaceutical University, Nanjing,China Medical School, Nanjing University, Nanjing 210002, C
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
418-421
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）