SIMILAR BUT NOT THE SAME: HCV F AND CORE PROTEIN ENHANCE C-MYC PROMOTER ACTIVITY
The F protein is expressed by a +1 ribosomal frameshift during HCV translation. Discovery of F protein expressed from the HCV core coding sequence challenges properties assigned to core protein. In this study, we aimed to investigate the functional role of F protein in transcriptional modulation of c-myc. Cells were cotransfected using Lipofectamine 2000 with a human c-myc-Luc reporter construct and different plasmid DNAs, pcDNA3-F or pcDNA3 - core. Luciferase assays were performed at 48h post-transfection to analyse changes in c-myc-Luc reporter construct expression. pcDNA3 - core and pcDNA3 - F enhanced luciferase activity about seven-fold and three-fold from the c-myc promoter, respectively. The enhancement of c-myc promoter was observed to be dose-dependent only by pcDNA3 - core, not pcDNA3 - F plasmid. We concluded that F protein do not posses all functions of core protein, at least in modulation of c-myc promoter.
Hepatitis C virus (HCV) F protein core protein c-myc promoter
Kong Jing
Faculty of Life Sceince & Chemical Engineering, Huaiyin Institute of Technology, Huaian 223300, China Medical Institute of Nanjing Army, Nanjing 210002, China
国际会议
The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)
北京
英文
434-437
2009-09-17（万方平台首次上网日期，不代表论文的发表时间）