STUDY ON THE ACTION MECHANISMS OF DNA POLYMERASES ON DNA SUBSTRATES USING CATIONIC CONJUGATED POLYMERS

摘要：

Cationic conjugated polymers (CCPs) are applied to study the function mechanisms of DNA polymerases by using fluorescence resonance energy transfer (FRET) technique. Our preliminary results show that two model DNA polymerases (Klenow fragment of DNA polymerase I and Taq DNA polymerases) have different interaction mechanisms with the DNA substrates and dNTPs, indicating the proposed CCPs-based FRET method may be promising to reveal the functional mechanisms of DNA polymerases.

关键词： FRET DNA polymerase conjugated polymer action mechanism

作者: Li Zhengping Zhang Xian Liu Xiuxian Liu Chenghui

作者单位: Key Laboratory of Medicine Chemistry and Molecular Diagnosis.Ministry of Education College of Chemistry and Environment Science, Hebei University, Baoding 071002, China

会议类型: 国际会议

会议名称: The 6th International Forum on Post-genome Technologies(6IFPT)(第六届国际后基因组生命科学技术学术论坛)

会议地点: 北京

会议语种:英文

页码: 441-443

在线出版日期: 2009-09-17（万方平台首次上网日期，不代表论文的发表时间）