Negative Effect of IFN-α2b against HBV-DNA in PBMC, Serum and Inducement to CD25 in the Patients with Chronic Hepatitis B
Objective: To study the effect of IFN-2b on HBVDNA and inducement to CD25 of the patients with chronic hepatitis B. Methods: All of patients were divided into two groups and treated by IFN-2b, routine medicine, respectively. The levels of HBV-DNA in PBMC and serum, and CD25 and CD25 mRNA, and anti-IFN-IgG were respectively detected by Real-time PCR, biotin-streptavidin (BSA) and ELISA. Results: After treatment for 24 and 48 weeks, the total negative rates of HBV-DNA in PBMC, serum and HBeAg were 36.36％, 39.39％, 40.91％ and 42.42％, 51.52％, 53.03％, respectively. There was significant difference between in the two groups (P<0.05～P<0.01). The level of CD25 either in silence or in inducement was low in chronic hepatitis B. Only was 4 (6.06％) cases with anti-IFN-IgG(+) and little discrepancy between in two groups (P>0.05). Conclusions: IFN-2b has better effect on HBV-DNA. CD25 can be induced by IFN-2b so that the active T cells play key role against HBVDNA in hosts. Low level of anti-IFN-IgG can be produced during the treatment.
WANG Jian SUN Lin XIANG Gui-ju HU Xiao-bin
Department of Aetiology, Anhui University of Science & Technology, Huainan 232001, China Department of Infectious Diseases, the Second Miner Hospital of Huainan, Huainan 232052,China Department of Infectious Diseases, the Second Miner Hospital of Huainan, Huainan 232052, China
国际会议
2009 IEEE International Symposium on IT in Medicine & Education( IEEE 教育与医药信息化国际会议)
济南
英文
1158-1164
2009-08-14（万方平台首次上网日期，不代表论文的发表时间）