Performance testing and analysis of electrophoresis process driven by low voltage on the SOI microchip

摘要：

In this paper, a new approach for amino acids separation on the electrophoresis chip based low voltage-driven electrophoresis was reported in detail. The insulation of the neighboring arrayed-electrodes was indicated by measuring breakdown voltage, which tested as 260V; by means of current monitoring method, the electroosmotic flow (EOF) of SOI-PDMS hybrid electrophoresis microchip was 3.9×10-4 cm2·V-1·s-1;the range of working voltages which selected by measuring the Volt-Ampere characteristic curve was 100-140·Vcm-1.Using the laser-induced fluorescence detector, a set of low-voltage driven electrophoresis separating and detecting system was finally established. Under the optimized conditions, the mixture of FITC-labeled arginine(Arg) and phenylalanine (Phl)was successfully separated with a resolution of 3.14 on the low-voltage driven electrophoresis microchip.

关键词： microfluidic chip low-voltage driven electrophoresis laser induced fluorescence amino acids separation

作者: LIANG Jing XU Yi HU Xiaoguo LU Jiali

作者单位: Key Disciplines Laboratory of Novel Micro-nano Devices and System Technology,Chongqing, 400030, P.R. Chemistry and Chemical Engineering College, Chongqing University, Chongqing, 400030, P.R.China

会议类型: 国际会议

会议名称: 第八届国际测试技术研讨会(8th International Symposium on Test and Measurement)

会议地点: 重庆

会议语种:英文

页码: 239-242

在线出版日期: 2009-08-01（万方平台首次上网日期，不代表论文的发表时间）