Association Analysis between Erythrocyte Membrane Surface Structure and Genes in Cyprinus carpio with Different Vitality at Lower Temperature
Erythrocyte membrane surface structure in common carp (Cyprinus carpio) with different vitalities at lower temperatures was observed using atomic force microscope (AFM). The results showed that carp individuals with cold tolerance have spine heave in its erythrocyte membrane edge, whereas the counterparts havent this structure. Two EST markers named of CC005 and CC062 isolated from cDNA library constructed under cooling temperature was found to be connected with the presence of spine heave in erythrocyte membrane. A significance (χ2=21.345, P＜0.01) was detected in genotypic frequencies related to the presence of spine heave obtained simultaneously at CC005 and CC062. Thus, we inferred that polymorphisms of two ESTs have a collaborative effect on the conformation of spine heave. This study will offer supportive theoretics to conduct blood physiological research of cold adaption in fish species.
Cyprinus carpio lower temperature erythrocyte membrane EST
Liqun LIANG Yumei CHANG Qingquan LEI
College of Electrical & Electronic Engineer, Harbin University of Science and Technology, Harbin, 15 Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070 College of Electrical & Electronic Engineer, Harbin University of Science and Technology, Harbin, 15
国际会议
哈尔滨
英文
1196-1201
2009-07-19（万方平台首次上网日期，不代表论文的发表时间）