Cloning and association analysis with cold adaptation of NADH-quinone ozidoreductase 3 subunit gene from common carp Cyprinus carpio

摘要：

Genes expressed specifically at cooling temperature were used to design primers and to screen brain full-length cDNA library of common carp (Cyprinus carpio) through two rounds of clonal PCR method. After that, gene 152 was discovered and sequenced, and determined finally as NADH-quinone oxidoreductase 3 subunit gene using homologous searches. This gene was confirmed to play an important role as the force generators for fish in a cooling water environment. In addition, NADH-quinone oxidoreductase 3 subunit gene was associated with cold adaptation of common carp to some extent in oxidation of respiratory chain electron transport system.

关键词： common carp Cyprinus carpio NADH-quinone ozidoreductase 3 subunit gene cloning cold adaptation

作者: Liqun LIANG Yumei CHANG Qingwei Zou Qingquan LEI

作者单位: College of Electrical & Electronic Engineering, Harbin University of Science and Technology, Harbin Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070 The College of Life Sciences, Nankai University, Tianjin 300071, CHINA College of Electrical & Electronic Engineering, Harbin University of Science and Technology, Harbin

会议类型: 国际会议

会议名称: 第九届电介质材料性能与应用国际会议(The 9th International Conference on Properties and Applications of Dielectric Materials)

会议地点: 哈尔滨

会议语种:英文

页码: 1202-1206

在线出版日期: 2009-07-19（万方平台首次上网日期，不代表论文的发表时间）