Biochip Based on Surface-Bound Cap-shaped Gold Nano-particles for Recognition of Biomolecules
In this paper, the optical biochips based on cap-shaped gold nano-particles have been constructed by evaporating gold on polystyrene and silica microspheres to form Au caps, which show a novel localized surface plasmon resonance (LSPR). The gold surface on silica spheres modified with a carboxymethyl dextran layer was used for sensing of anti-avidin D with a detection limit of 5 nmol·L-1 and a sensitivity of 3.4 × 10.3 nm·L·nmol-1, which was based on surface-bound avidin molecule. Furthermore, a double stranded DNA (dsDNA) with a binding site of hexa sequence AGGTCA for a nuclear receptor, retinoid X receptor beta (RXRβ), has been successfully immobilized on gold surface via a specific interaction between biotin moiety and streptavidin, and applied to selective recognition of small molecule nuclear ligands like 9-cis retinoic acid.
Optical biochip Cap-shaped Gold nano-particle Biomolecule recognition LSPR
Z.Cao S.X.Wu F.C.Gong L.Zhang Y.L.Yin
School of Chemistry and Biological Engineering, Changsha University of Science andTechnology, Changs School of Chemistry and Biological Engineering, Changsha University of Science andTechnology, Changs Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, P.R.China
国际会议
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)
广州
英文
55-59
2009-07-04（万方平台首次上网日期，不代表论文的发表时间）