Developmental Ezpression of N-acetylglutamate synthase in Tibetan porcine Intestine During Lactation

摘要：

N-acetylglutamate synthase (NAGS) catalyzes the formation of N-acetylglutamate (NAG),which is an obligatory allosteric activator of carbamylphosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. In order to better nuderstanding the developmental expression of intestinal NAGS in Tibetan piglets during lactation. We cloned the gene of NAGS from Tibetan piglets, which encoded 368 amino acid residues showing a higher degree of sequence similarity with the “conserved domain of human and the mouse NAGS. NAGS gene was successfully expressed in Escherichia coli BL21 and the rabbit antiserum against the purified NAGS was made. The expression levels of NAGS mRNA and protein were generally decreased in jejunum and ileum from day 7 to 35 (linear, P < 0.05 and P < 0.01 respectively). As decreasement of NAGS expression may lead to intrinsically synthesis of arginine decline, on the molecular basis for age-related alterations of Tibetan porcine NAGS activity in the intestine, regulation of Tibetan porcine NAGS expression is gateway to increase the intestinal endogenous synthesis of arginine in piglet.

关键词： N-acetylglutamate synthase Tibetan pigs Developmental ezpression

作者: M.M.Geng W.Y.Chu T.J.Li W.C.Wang W.T.Gu C.Y.Shi R.L.Huang Y.L.Yin G.Y.Wu

作者单位: Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of SubtropicalAgricult Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of SubtropicalAgricult Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agricul

会议类型: 国际会议

会议名称: 4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)

会议地点: 广州

会议语种:英文

页码: 105-114

在线出版日期: 2009-07-04（万方平台首次上网日期，不代表论文的发表时间）