Dietary L-arginine supplementation can increase the vascular endothelial growth factor(VEGF) and HSP70 ezpression in weaned pigs
This study was conducted to evaluate the effects of dietary arginine on expression of VEGF and HSP70 in weaned pigs. Twenty 21-d-old crossbred pigs (5.3 ± 0.13 kg body weight) were individually housed and randomly allotted to 1 of 2 diets supplemented with 0 and 1％ L-arginine (10 pigs per group). Following a 7-d period of arginine supplementation, 6 pigs from each group were randomly selected and humanely killed for tissue sample collection. The expression of VEGF in intestine was determined by immuno-histochemistry and HSP70 in liver was tested by real-time PCR, western blot and immuno-histochemistry. Compared with control pigs, dietary supplementation with 1％ L-arginine increased (P < 0.05) average daily gain (ADG) and decreased (P < 0.05) feed/gain ratio. Supplementation with 1％ L-arginine increased (P <0.05) the villus height throughout the small intestine compared with the controls. Crypt depth was greater in the duodenum and jejunum of the control group compared with those supplemented with 1％ L-arginine (P<0.05). Dietary supplementation with 1％ L-arginine also increased (P<0.05) immunoreactive expression of vascular endothelial growth factor (VEGF) in duodenal submucosa, jejunal mucosa and submucosa, and ileal mucosa, and also increased HSP70 mRNA expression in liver (P<0.05) compared with the control. Collectively, these findings indicate that arginine supplementation can improve the growth of weaned pigs by enhancing intestine development and ability of anti-stress. Moreover, dietary supplementation with 1％ L-arginine may be a useful method to improve growth performance of weaned pigs.
L-arginine weaned pigs growth performance vascular endothelial growth factor (VEGF) HSP70
K.Yao M.Fan G.Y.Wu J.Pan R.J.Chen T.J.Li M.M.Geng R.L.Huang Y.L.Yin
Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of SubtropicalAgricult Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agricul
国际会议
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)
广州
英文
115-125
2009-07-04（万方平台首次上网日期，不代表论文的发表时间）