Ezpression profiling of transporter genes in swine conceptus trophectoderm cells with arginine supplementation using a swine microarray1
Intrauterine growth retardation (IUGR) is a major concern in domestic animal production. Because nutrient transfer across the placenta is essential for fetal growth and development, as well as a placental transport defect in IUGR, we profiled the expression of transporter genes in swine conceptus trophectoderm cells with arginine (arg) supplementation using a swine microarray in this study. Our results showed that there were 7 (3.6％) and 20 (10.4％) higher expression genes, 41 (21.2％) and 38 (19.7％) lower expression genes, and 145 (75.1％) and 135 (69.9％) no expression genes in 100 and 350 μM arg supplemented groups, respectively. Supplementation of 100 μM arg in the medium up-regulated the expression of sodium/glucose cotransporter, member 9 of solute carrier family 16 (monocarboxylic acid transporters), choline transporter-like protein 2,glycine betaine/L-proline ABC transporter, and amino acid system A transporter, while down-regulated the expression of ABC cobalamin/Fe3+-siderophore transporter, when compared with 350 μM arg group. These regulating activities of arg to transporter genes might be related with its improving the survival of porcine embryos/fetuses and enhancing the nutritional provision to the fetuses for supporting their in utero growth.
Transporter gene Conceptus trophectoderm Swine Microarray
X.F.Kong G.Y.Wu Y.Wang H.J.Zhou Y.L.Yin B.E.Tan X.L.Li T.E.Spencer H.J.Gao
Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of SubtropicalAgricult Department of Poultry Science, Texas A&M University, College Station, TX 77843, USA Key Laboratory for Agro-ecological Processes in Subtropical Region, Institute of SubtropicalAgricult Department of Animal Science, Texas A&M University, College Station, TX 77843, USA
国际会议
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)
广州
英文
273-289
2009-07-04（万方平台首次上网日期，不代表论文的发表时间）