Methodological Comparison for Determination of the Duodenal Flows of Endogenous Nitrogen and Amino Acids in Growing Goats
The objective of the present study was to compare the isotope dilution, the difference and the amino acid profile (AAP) methods for quantification of the duodenal flows of endogenous nitrogen (EN) and endogenous amino acids (ENAA) in growing goats. Nine growing goats were fed the same diet containing maize stover, ground corn and soybean meal. The duodenal flow of EN determined by the isotope dilution method was significantly (P ＜ 0.01) greater than that determined by the difference and AAP methods. The duodenal flows of ENAA determined by the isotope dilution method were 1-fold greater than those determined by the difference and the AAP methods. It is concluded that the difference and the AAP methods underestimate the duodenal flows of EN and ENAA compared to the isotope dilution method.
Isotope dilution method Difference method Endogenous nitrogen Endogenous amino acids Goats
C.S.Zhou Z.L.Tan J.R.Wang Y.F.Pan S.X.Tang Z.H.Sun X.F.Han M.Wang S.M.Liu
Key Laboratory of Agro-ecological System Processes of Subtropical Region, Institute ofSubtropical Ag Key Laboratory of Agro-ecological System Processes of Subtropical Region, Institute ofSubtropical Ag The University of Western Australia, Perth 6009, Australia
国际会议
4th International Symposium on Animal Nutrition,Health and Feed Additive(第四届动物营养、保健与饲料添加剂国际学术研讨会)
广州
英文
373-383
2009-07-04（万方平台首次上网日期，不代表论文的发表时间）