Biochemical and Molecular Properties of a P Starvation Induced APase Isoform, PvPAP3, in Common Bean (Phaseolus Vulgaris L.)
Enhanced acid phosphatase (APase) activities under phosphorus (P) deficient conditions have been proved to play an important role in P homeostasis of plants. However, molecular and biochemical properties of P starvation induced APase is not well studied. In the present study, APase isoform expression patterns as related to P availability were compared in two common bean genotypes contrasting in P efficiency. One P starvation induced APase isoform was purified and sequenced. Subsequently, PvPAP3, encoding the APase isoform was isolated through PCR amplification from the cDNA library. Activity of PvPAP3 against tartrate inhibition, along with the cDNA full length sequences, indicated PvPAP3 is a purple APase (PAP). Semi-quantitative RT-PCR results showed that the expression level of PvPAP3 was specifically increased by P starvation and depended on P availability. Furthermore, the expression level of PvPAP3 in C19833, a P efficient genotype was stronger than that in Dor364, a P inefficient genotype, indicating that PvPAP3 might contribute to high P efficiency in common bean.
Cuiyue Liang Jiang Tian Xiaolong Yan Hong Liao
Root Biology Center, South China Agricultural University, Guangzhou 510642, China
国际会议
The 7th International Symposium on Plant-Soil Interactions at Low pH(第七届低pH条件下植物-土壤交互作用国际研讨会)
广州
英文
65-66
2009-05-17（万方平台首次上网日期，不代表论文的发表时间）