ESR Measurement of Radical Reactive Ozygen Species in Soybean Roots in the Response to Aluminum Stress
5-Diethoxyphosphoryl-5-methyl-l-pyrroline-N-oxide (DEPMPO) was used as spin trapping reagent to identify radical ROS in Al-treated soybean roots. In addition to hydroxyl radical adduct, unidentified signals were observed. As a model study, liposome of phosphatidylcholine derived from soybean was incubated with hydroxyl radical. The ESR spectrum was almost identical to that of the root homogenate, indicating that the unidentified signals may be originated from a carbon-centered radical derived from lipid. ESR spectra of Al-treated root showed a consistently increased signal intensity of the unidentified signals. It can be concluded that Al induces hydroxyl radical, which may be converted to the lipid-derived radical.
Mitsuru Abo Hiroki Yonehara Etsuro Yoshimura
Department of Applied Biological Chemistry, School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan
国际会议
The 7th International Symposium on Plant-Soil Interactions at Low pH(第七届低pH条件下植物-土壤交互作用国际研讨会)
广州
英文
128-129
2009-05-17（万方平台首次上网日期，不代表论文的发表时间）