Recent Advances in Agrobacterium Rhizogenes Mediated Transformation for Root Biology Studies
Agrobacterium rhizogenes mediated transformation has been extensively used in dicots to produce transformed hairy roots. Different transformation protocols have been developed for hairy root transformation. These protocols vary from their complexity to simplicity for the production of transformed hairy roots to study root biology, microbial interactions, and functional analysis of genes using over expression or suppression of genes. The difference between hairy root transformation protocols is mainly based upon the type of explants and subsequently the growth conditions but sonication and acetosyringone have been also used to enhance A. rhizogenes efficiency ( Kumar et al. , 2006 ). There are three major ways for the production of transformed hairy roots as described below.
Muhammad Sameeullah Xiurong Wang Hong Liao Xiaolong Yan
Root Biology Center, South China Agricultural University, Guangzhou 510642, China
国际会议
The 7th International Symposium on Plant-Soil Interactions at Low pH(第七届低pH条件下植物-土壤交互作用国际研讨会)
广州
英文
157-158
2009-05-17（万方平台首次上网日期，不代表论文的发表时间）