Construction of Stylosanthes Guianensis Suppression Subtractive Hybridization Library under Aluminum Stress
Stylo (Stylosanthes quianensis) is a worldwide-grown forage grass and a cultivar of Stylo ( cv. Reyuan No. 2) was shown to exudate citrate and tolerate to Al. In order to understand the mechanism of Al detoxification in stylo plants at molecular level, a cDNA library for Reyuan No. 2 under Al stress was constructed by suppression subtractive hybridization ( SSH). Ultimately, 1300 randomly selected positive clones were obtained and consisted of the SSH cDNA library. Approximately 600 clones were randomly selected for sequence analyses. The results indicated that many previously reported stress-related genes were included in this SSH library.
Qifeng Wang Diqui Liu Qiong Yi Kunzhi Li Yongxiong Yu Limei Chen
Biotechnology Research Center, Kunming University of Science and Technology, Kunming, China College of Zoological Science and Technology, Southwest University, Chongqing, China
国际会议
The 7th International Symposium on Plant-Soil Interactions at Low pH(第七届低pH条件下植物-土壤交互作用国际研讨会)
广州
英文
159-160
2009-05-17（万方平台首次上网日期，不代表论文的发表时间）