miR-292-5p,let-7g Regulating Protein Kinase C ,Effecting β-casine Ezpression in Murine Mammary Epithelial Cell
The mammary gland has the ability to undergo repeated cycles of tightly regulated postnatal proliferation, differentiation, and apoptosis-mediated regression, providing a model to investigate potential regulators of mammary epithelial growth and differentiation. Protein kinase C forms a central control point mammary gland activated cell-signalling events and regulate a wide of intracellular function including induction of β-casein expression. In this study miR-292-5p,let-7g were inhibited in the mouse mammary epithelial cell. By Western blot analysis of whole cell lysates from purified MEC knock down miR-292-5p or let-7g , PKC protein expression was found upregulating. Bioinformatic predictions suggest that the mouse PKC mRNA 3-untranslated region (3-UTR) contains two miR-292-5p target sites, two let-7g target sites. As well as β-casine expression was change with knock down miR-292-5p or let-7g.Conclusion miR-292-5p , let-7g play a role in mammary cell differentiation and mediates the effects of β-casein expression by target PKC gene.
ChunMei Wang QingZhang Li Xuejun Gao
Key laboratory Dairy Science of Education Ministry Northeast Agricultural University,Harbin, China
国际会议
奶牛营养与牛奶质量国际研讨会(ist International Symposium on Dairy Cow Nutrition and Milk Quality)
北京
英文
29-35
2009-05-04（万方平台首次上网日期，不代表论文的发表时间）