Based on quantum dots detection and immuno-fluorescence imaging of anti-nuclear antibody in different tissue cells
(0) To evaluate the effectiveness of Quantum dots (QDs) labeled antibody probe in the applications of indirect immunofluorescence assay (IIFA). IIFA was used for ANA detection based on QD655-tags, assay results which generated were also compared with that of FITC tags. There existed results discrepancies at a certain degree between QD655-and FITC-tags, including centromere pattern, nuclear homogeneous pattern, nucleolar pattern and cytoplasmic granular pattern, etc, which generated by the target antigen positioned in different parts of the cells. QD655-tags was priority with cytoplasm antigen/antibody reaction, but weakly stained with interphase-cell nuclei, nuclear centromere, dsDNA, nucleosome and histone nuclear-antigens relatively. Yet, ANA results generated by QD655 and FITC fluorescent labellings were roughly approached conformity in split-phase cells. Based on QD-tags, IIFA used for ANA detection is feasible, and has advantages of sensitivity and stability. Synchronously, QD-and FITC-tags together used for ANA will achieve the better effectiveness.
quantum dot anti-nuclear antibody indirect immunofluorescence assay fluorescein isothiocyanate
Yu Wei Li Xiao-Jun Zhang Yu Luo Bing Fang Guo-Xiang Bai Cai-Qin Wu Jian-Guo Gu Ning
Research Institute of Clinical Laboratory Medicine, Nanjing Jinling Hospital, Medical School, Nanjin Key Laboratory of Biomaterials and devices of Jiangsu Province, Southeast University, Nanjing 210096
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
36-39
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）