Biological activity assays and cellular imaging of anti-human sperm protein 17 immunomagnetic nanoparticles
Sperm protein 17 is a novel cancer-testis antigen and can be candidated as a new target for cancer immunotherapy and molecular diagnosis. The anti-human Sperm protein 17 immunomagnetic nanoparticles were prepared by grafting anti-human Sperm protein 17 antibodies on the surface of chitosan-coated magnetic nanoparticles using the linker of 1-ethyl-3-3-dimethylaminopropyl carbodiimide/N-hydroxysuccinimide. The morphology and properties of the nanoparticles were characterized by transmission electronic microscopy, the conjugation of the antibodies was evaluated by native-polyacrylamide gel electrophoresis, the immunologic activity of the particles was evaluated by enzyme linked immunosorbent assay. A set of in vitro magnetic resonance imaging experiments were performed after incubated the immunomagnetic nanoparticles with human Sperm protein 17 gene transfected ovarian cancer HO-8910 cells. The results showed that we successfully grafted the magnetic nanoparticles with anti-Sperm protein 17 antibody and the IMNPs kept good bioactivity. And the immunomagnetic nanoparticles were targeted successfully to the positive cells, and no obviously nonspecific adsorption was observed.
immunomagnetic nanoparticles sperm protein 17 activity assays magnetic resonance imaging
Liu Qun Ge Yu-Qing Li Fang-Qiu Zhang Shi-Xin Gu Ning
Institute of Medical Laboratory Sciences, Nanjing Jinling Hospital, Medical School, Nanjing Universi Department of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, Chi
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
40-43
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）