Chemical synthesis of follicle-stimulating hormone gene and stable ezpression in CHO cells

摘要：

Based on amino acids sequences of a subunit and β subunit of follicle-stimulating hormone (FSH), gene sequence was optimized which is easy to be highly expressed in eukaryotic cells, and were chemically synthesized. With genetic engineering techniques, α subunit and β subunit genes were cloned into two eukaryotic cell expression vectors PM902 and pcDNA3.1( + ) respectively, and co-transfected into CHO cell lines for construct stable and high expression cell lines. The expressed human FSH can be applied for therapy of infertility, nurture of zygotes in vitro, preparation of polyclonal antibody and monoclonal antibody to detect human FSH and so on.

关键词： follicle-stimulating hormone ezpression CHO

作者: Li Yue-Xi Li Su-Qin Pan Ying Pan Ming-Jie

作者单位: The East-China Institute of Medical Biotechniques, The Key Laboratory of Medical Biotechniques of Ji The East-China Institute of Medical Biotechniques, The Key Laboratory of Medical Biotechniques of Ji

会议类型: 国际会议

会议名称: International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)

会议地点: 南京

会议语种:英文

页码: 44-47

在线出版日期: 2009-04-27（万方平台首次上网日期，不代表论文的发表时间）