Construction of a yeast ezpression vector of Hepatitis C Virus Non-structural 5B gene

摘要：

To construct the yeast expressing HCV NS5B protein with C-terminal 21 amino acids truncated and to acquire the protein for screening the anti-HCV drugs targeting the NS5B. The coding region of HCV NS5B-C21 gene was amplified by PCR and was digested by Xho I and Not I , the gene fragment was cloned into plasmid pPICZα. The positive recombinant plasmid was obtained by identification of PCR amplification; it was named pPICZα-NS5B-C21. Emzymic hydrolysis verified that NS5B-C21 gene was correctly cloned to the vector. The successful constructing of yeast expression plasmid containing NS5B-C21 lay a better basis for carrying out further studies on establishing foundation for screening the anti-HCV drugs targeting the NS5B.

关键词： hepatitis C virus non-structural 5B gene pPICZα

作者: Li Lin Wang Zheng-Mao Pan Ming-Jie Li Yue-Xi

作者单位: Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, 210029,China East-China Institute for Medical Biotechniques, Nanjing, 210002, China

会议类型: 国际会议

会议名称: International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)

会议地点: 南京

会议语种:英文

页码: 58-60

在线出版日期: 2009-04-27（万方平台首次上网日期，不代表论文的发表时间）