Detect antibody of enolase using mimical antigen for diagnosis of invasive Candadiasis
The diagnosis of invasive candidiasis (IC) was attempted by detection of circulating antibody against enolase by an ELISA assay established using the mimical antigen selected from phage display, and this method was compared with the conventional culture method. One hundred and thirty sera from patients with various Candida infection confirmed by microbiological culture were collected. The surrogate peptides were used for coating antigen to detect antibodies against enolase in the patients sera, and then the method was evaluated for distinguishing between invasive and superficial infection of C. albicans, and the cross reaction with other Candida infection. The antibody was detectable in all of 35 patients with invasive infection of C. albicans, 2 out of 42(4. 8％) patients with superficial infection of C. albicans and 3 out of 18 (16. 7％) patients with invasive infection of C. tropicalis. The enolase antibody was negative for sera from patients infected with C. glabrata, C. parapsilosis and un-identified Candida, no matter invasive or superficial.
Candida albicans mimic antigen ELISA invasive infection
Li Fang-Qiu Zhou Wan-Qing Shao Hai-Feng Wang Wei-Ping Shi Li-Ning
Institute of Medical Laboratory Sciences, Nanjing Jinling Hospital, Medical School, Nanjing University, Nanjing, 210002,China
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
64-67
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）