Establishment of Stable Focal adhesion kinase(FAK) RNA Interference Cell Line
Malignant melanoma continues to remain a significant health threat, with death often occurring as a result of metastasis. Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase implicated in cell cycle progression and cell migration. Overexpression of FAK in a variety of tumors has suggested that FAK is a promising target for therapeutic intervention. In our previous study, we have demonstrated that plasmid-encoded FAK small interfering RNA (siRNA) dramatically inhibited in vitro mouse melanoma cell line B16F10 proliferation and invasion. Here, to further investigated the functional role of FAK, we repressed FAK expression by stable transfection of plasmid-encoded FAK small interfering RNA (siRNA). This stable cell line will be used to investigate the molecular mechanisms that promote an aggressive phenotype of melanoma.
FAK stable transfection B16F10 siRNA
Lan Yan Tang Bo Hua Zi-Chun
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, China
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
77-79
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）