Ezpression and purification of a novel soybean seed ferritin H-5/H-2 complez

摘要：

A novel soybean seed ferritin gene, SferH-5, was firstly isolated from 7-day-old soybean seedlings. We speculate that SferH-5 encodes a novel 26. 5-kDa subunit of soybean seed ferritin, which is designated H-5. After the target fragment was confirmed by DNA sequencing, the gene sferH-2 and sferH-5 were correctly inserted into the prokaryotic expression plasmids vector pET28a and the yield recombinant plasmid was transformed to the E. coli BL21 (DE3). H-2/H-5 complex was successfully expressed in E. coli and collected by affinity chromatography with Ni2+ column.

关键词： ferritin H-5/H-2 complez pET28a BL21 (DE3) Ni2+ column

作者: Zhang Xiang-Yu Li Jie Dong Xiang-Bai Hua Zi-Chun

作者单位: The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, 210093, China

会议类型: 国际会议

会议名称: International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)

会议地点: 南京

会议语种:英文

页码: 84-86

在线出版日期: 2009-04-27（万方平台首次上网日期，不代表论文的发表时间）