Generation and characterization of recombinant neutralizing antibody Fab against rabies virus

摘要：

Passive immune therapy and the using of rabies vaccine were considered to be an important part of blocking rabies virus. Some single chain antibodies (scFv) against rabies virus were selected by phage display techniques. The purpose of this study is to transform the scFv into Fab, and identify the biologic activity. The variable region genes, VH and VL, were amplified by PCR from anti-rabies virus scFv plasmids. The Fab gene was inserted into vector pcomb3XSS and transformed into E. coliTop10 for a great deal of soluble expression and purification. The Fab was purified by IMAC with FPLC system, and detected by western blot and indirect ELISA. Meanwhile, the fluorescent antibody virus neutralisation test (FAVN) showed Fab hasa high neutralizing activity against rabies virus strain CVS-11. This Fab fragment can be further modified to generate a full-length antibody for rabies therapy.

关键词： human Fab fragment neutralizing antibody soluble ezpression

作者: Li Chen Guan Xiao-Hong Zhu Jin Liu Xin-Jian Wang Zhong-Can Lin Hong Pan Ying Li Su-Qin Li Yue-Xi Feng Zhen-Qing

作者单位: Key Laboratory of Antibody Technique of Health Ministry, Nanjing Medical University, Nanjing, 210029 Huadong Medical Institute of Biotechniques, Nanjing, 210002, China

会议类型: 国际会议

会议名称: International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)

会议地点: 南京

会议语种:英文

页码: 94-97

在线出版日期: 2009-04-27（万方平台首次上网日期，不代表论文的发表时间）