Identification of a histidine triad protein family protein from Streptococcus suis 2
(0) A whole genome sequence analysis of the Chinese strain 052YH33 of S. suis 2 enabled us to identification of a novel histidine triad protein gene. The 2.7 kb target DNA fragment was successfully amplified and the recombinant HtpS (histidine triad protein of Streptococcus suis) can be produced in E. coli under the induction of IPTG. A 110 kDa protein was purified by affinity chromatography. Western-blot experiment demonstrated the recombinant protein shares strong specific antigenicity. Flow cytometry analysis demonstrated that the protein can be exposed on the surface of 05ZYH33 strain. Animal protection experiments demonstrated that immunizing with HtpS could protect mice against lethal doses of bacteria which suggested the protein could apply as a vaccine candidate.
Streptococcus suis serotype 2 (S. suis 2) HtpS surface protein vaccine candidate
Shao Zhu-Qing Pan Xiu-Zhen Han Ming-Yue Zheng Feng Tang Jia-Qi
College of Life Science, Nanjing Normal University, Nanjing, 210046, China Huadong Research Institit Huadong Research Institite for medicine and Biotechnic, Nanjing, 210002, China
国际会议
International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)
南京
英文
103-105
2009-04-27（万方平台首次上网日期，不代表论文的发表时间）