Mitochondria-dependent apoptosis in HeLa cell induced by methylene blue-mediated photodynamic therapy

摘要：

Methylene blue (MB), a widely studied reagent, is investigated in this work for its usage in photodynamic therapy (PDT). PDT has been proved to be highly effective in the treatment of different types of cancers. To elucidate the effects of MB in PDT, we analyzed PDT-induced apoptosis in HeLa cells by introducing different doses of MB into the culture media. Our data showed that MB-mediated PDT triggered intense apoptotic cell death beginning with photochemical generation of reactive oxygen species. The release of cytochrome c and activation of caspase-3 indicated that MB-PDT-mediated apoptosis in HeLa cells was executed by the mitochondria-dependent apoptotic pathway.

关键词： apoptosis methylene blue HeLa cell photodynamic therapy mitochondria

作者: Lu Yan Chen Xiao-Ping Shen Ping-Ping

作者单位: State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210093, China

会议类型: 国际会议

会议名称: International Symposium on Medical and Pharmaceutical Biotechnology(医药生物技术国际研讨会)

会议地点: 南京

会议语种:英文

页码: 112-115

在线出版日期: 2009-04-27（万方平台首次上网日期，不代表论文的发表时间）